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| Format: | Artículo científico |
| Language: | en |
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Universidad de Córdoba
2019
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| Online Access: | https://www.redalyc.org/articulo.oa?id=69360322012 https://www.redalyc.org/journal/693/69360322012/ https://www.redalyc.org/journal/693/69360322012/html/ https://www.redalyc.org/journal/693/69360322012/69360322012.epub https://www.redalyc.org/journal/693/69360322012/movil https://doi.org/10.21897/rmvz.1412 |
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Table of Contents:
- Use of disaccharides and activated carbon to preserve cellulolytic ruminal bacterial consortiums lyophilized Javier Texta Nogueda Paulino Sánchez-Santillán David Hernández Sánchez Nicolás Torres-Salado María Crosby Galvan Rafael Rojas-García Jerónimo Herrera-Pérez María Maldonado-Peralta Veterinaria lactose preservatives In vitro fermentation lyophilization maltose Objective. To determine in vitro fermentation of cellulolytic ruminal bacterial consortia (CBC) preserved by lyophilization using activated carbon, maltose and lactose as preservatives. Materials and methods. A CBC was isolated from the ruminal fluid of a female water buffalo in selective cellulolytic media. The CBC were lyophilized without preservative (SP), activated carbon (CA), lactose (LA) o maltose (MA) as preservatives. The experimental design was completely random to measure biogas at different time intervals; as well as completely random with 4x3 factorial arrangement, factors were preservative SP, CA, LA and MA and fermentation time (24, 48 and 72 h) for pH, ammoniacal nitrogen (NH3-N), dry matter degradation (DMD), neutral detergent fiber degradation (NDFD), enzymatic activity cellulases and total bacteria population. Results. LA produced higher accumulated biogas at 72 h and partial biogas after 12 h (p≤0.05). SP did not show differences (p>0.05) in cellulases, total bacteria population, DMD and NDFD in the fermentation times evaluated with the rest of the preservative. Conclusions. The production of partial and accumulated biogas, the increase in the degradation rate of 8.3 and 91.1% in the DMD and NDFD from 24 to 72 h (p≤0.05) in the LA preservative, show that lactose can be used as a preservative of ruminal cellulolytic bacteria. 2019 artículo científico 0122-0268 https://www.redalyc.org/articulo.oa?id=69360322012 https://www.redalyc.org/journal/693/69360322012/ https://www.redalyc.org/journal/693/69360322012/html/ https://www.redalyc.org/journal/693/69360322012/69360322012.epub https://www.redalyc.org/journal/693/69360322012/movil https://doi.org/10.21897/rmvz.1412 en http://www.redalyc.org/revista.oa?id=693 Revista MVZ Córdoba application/pdf Universidad de Córdoba Revista MVZ Córdoba (Colombia) Num.3 Vol.24